Experimental hemolytic anemia in rabbits; protective role of sensitization to the species-specific protein of the heteroimmune hemolytic serum.

By ISRAEL DAVIDSOHN, DAVID HERMONI AND ELEANOR G. HANAWALT H EMOLYTIC ANEMIA was one of the first diseases of blood produced artificially and studied in animals. Much valuable information has been gathered in this way. However, some moot points remain which require elucidation. In this paper experiment-s will be described which add to elucidation of the nature of a hitherto not fully understood phenomenon in the natural history of experimental hemolytic anemia. The standard procedure for the production of experimental heteroimmune hemolytic anemia is t.o select animals of two different species; one for the actual hemolytic experiment, the other as the source of hemolytic serum. Various systems have been employed by various authors since 1898, when Belfanti and Carhone introduced t-he use of heterologous immune serum for the study of experimental hemolyt-ic anemia in animals.tm Dameshek and Schwartz st.immilated renewed interest in this field by reporting a systematic study of t-he experimental disease, including minute technical details, and by pointing out the similarity with human disease.2 Their studies have thrown much light on the disease in animals and in man. In the present study, heteroimmune hemolytic serum was produced in guinea pigs injected with erythrocytes of rabbits. The hemolytic serum produced in this manner was then imiject-ed int.o rabbits.